Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc HBS-P+ Buffer (10X, pH 7.4) - 4x500ml
HBS-P Plus Buffer (10X pH 7 4) - 4x500ml (For Research Use and Further Manufacturing Only)
Sigma Aldrich Fine Chemicals Biosciences Hanks Balanced Salts Modif1L
HANKS Hanks Balanced Salts Modif1L
Teknova 1M Tris-HCl, pH 8.0. 1000mL, Sterile, Endotoxin Tested, PCR Grade.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
This DMEM-Hi glucose medium is a 1x complete medium with no added factors (common modifications) such as L-alanyl-L-glutamine HEPES or sodium pyruvate. It differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.
Teknova 1X PBS. 1000mL, Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Research Products International Corp DPBS 1X Solution, 500 Milliliters
Phosphate Buffered Saline is a buffer solution commonly used in biological research. PBS has many uses because it is isotonic and non-toxic to most cells. These uses include substance dilution and cell container rinsing.
Ready to use sterile 1X solution.
Ingredients:
Potassium Chloride: 2.7 mM
Phosphate Buffer: 11.9 mM
pH: 7.4
Does not contain Calcium or Magnesium
Teknova 200mM Tris, pH 7.0. 1mL, Sterile. 50 Pack
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
eMolecules 7365-44-8 | TES | Oakwood Chemical | MFCD00007532 | 229.250 | C6H15NO6S | 99.000 | OCC(CO)(CO)NCCS(O)(=O)=O | 1g | 537724648
TES | Oakwood Chemical | 7365-44-8 | MFCD00007532 | 229.250 | C6H15NO6S | 99.000 | OCC(CO)(CO)NCCS(O)(=O)=O | 1g | 537724648
Quality Biological Inc PBS pH 7.4 without Calcium and Magnesium, 20 Liters
PBS (phosphate buffered saline) ¿ Balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. ¿ Endotoxin and Mycoplasma tested ¿ Applications: immunological applications, such as Western Blotting. ¿ Storage Condition: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Endotoxin and Mycoplasma Tested ¿ pH: 7.3 ¿ 7.5 ¿ Osmolality: 300¿5% mOsm/Kg H2O ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 12161700 ¿ ¿ Max Shelf Life: 24 months
Quality Biological Inc DPBS without Calcium and Magnesium, 4 Liters
DPBS is a balanced salt solution (BSS) ¿ Used for the handling and culturing of mammalian cells. ¿ Calcium and magnesium facilitate cell binding and clumping. ¿ DPBS without these ions can be used to wash and rinse suspended cells. ¿ Applications: DPBS serves as an irrigating, transporting or diluting fluid for mammalian cells, while maintaining cell tonicity and viability for a limited period of time. ¿ Used as a diluent of fresh red blood cells to study the trapping of red blood cell aggregate Liters ¿ Wash the wells of microtiter plates ¿ Wash cells during preparation and serial transfer. ¿ Storage Condition: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 12161700 ¿ ¿ Max Shelf Life: 24 months