Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![eMolecules 1214727-57-7 | tert-Butyl (trans-3-(aminomethyl)cyclobutyl)carbamate | Ambeed | MFCD22123684 | 200.282 | C10H20N2O2 | 98.000 | CC(C)(C)OC(=O)N[C@H]1C[C@H](CN)C1 | 1g | 575777276](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000158799.jpg-250.jpg)
eMolecules 1214727-57-7 | tert-Butyl (trans-3-(aminomethyl)cyclobutyl)carbamate | Ambeed | MFCD22123684 | 200.282 | C10H20N2O2 | 98.000 | CC(C)(C)OC(=O)N[C@H]1C[C@H](CN)C1 | 1g | 575777276
tert-Butyl (trans-3-(aminomethyl)cyclobutyl)carbamate | Ambeed | 1214727-57-7 | MFCD22123684 | 200.282 | C10H20N2O2 | 98.000 | CC(C)(C)OC(=O)N[C@H]1C[C@H](CN)C1 | 1g | 575777276
Bioworld TT Buffer, Ready-Pack (10X), 2 L
Tris-Tricine BufferTris-Tricine (TT) Buffer has been developed for both non-denaturing and denaturing electrophoresis of proteins, and offers several advantages over more conventionally used buffers. TT Buffer allows for optimal resolution of low molecular weight proteins and elimantes the glycine front often found in gel utilizing TG-SDS and other such beffers. This improved running buffer allows for resolution of peptides as low as 5kD. TT buffer is also excellent for protein electrophoresis where resolved products will be transferred to a nylon membrane. Unlike glycine, tricine will not interfere with membrane protein sequencing. The 10X pack just requires the addition of distilled H2O to make 1.0 Liter of 10X liquid. A simple 10-fold dilution with water provides a 1X solution, that makes an excellent running buffer for most applications. Technical Information:Ultra PureGradeStorage: RTHazard: Irritant
eMolecules 1184985-22-5 | tert-butyl 3-(hydroxymethyl)-4-methyl-pyrrolidine-1-carboxylate | MFCD08461237 | 1g
ChemScene | 3-Hydroxy-5-(trifluoromethyl)benzonitrile | 100mg | 712788143 | CS-0373293 | 1243459-56-4 | MFCD16999150 | 187.121 | C8H4F3NO
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eMolecules 1378888-43-7 | 1-(3-Ethyl-4-(hydroxymethyl)phenyl)ethanone | MFCD18910891 | 1g
Ambeed | Methyl 1234-tetrahydroquinoline-6-carboxylate | 100mg | 490562747 | A465033 | 177478-49-8 | MFCD08692534 | 191.230 | C11H13NO2
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![Alkali Scientific TBE 10X Solution [TRIS borate EDTA 10X Solution], 4 Liter Cube Container](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661290.jpg-250.jpg)
Alkali Scientific TBE 10X Solution [TRIS borate EDTA 10X Solution], 4 Liter Cube Container
TBE 10X Solution is a highly effective electrophoresis buffer solution, formulated with Tris, borate, and EDTA. This 4-liter cube container provides a concentrated 10X solution that is commonly used for gel electrophoresis of nucleic acids. TBE helps maintain a consistent pH and ionic strength, critical for the accurate migration of DNA and RNA during electrophoresis. The 10X concentration allows for easy dilution to a working concentration of 1X, making it an ideal buffer for high-throughput applications in molecular biology. This large container is perfect for laboratories that perform a high volume of gel electrophoresis and other nucleic acid analysis procedures.
Sigma Aldrich Fine Chemicals Biosciences BICINE | 150-25-4 | MFCD00004295 | 25g
BICINE | Purity: ≥99% (titration) | MW:163.17 | 150-25-4 | MFCD00004295 | 25g
Sigma Aldrich Fine Chemicals Biosciences CAPS anhydrous, free-flowing, Redi-Dri(TM), >=99% | 1135-40-6 | MFCD00003837 | 500G
CAPS anhydrous, free-flowing, Redi-Dri(TM), >=99% | Purity: >=99% | Mol Wt: 221.32 | 1135-40-6 | MFCD00003837 | 500G
Sigma Aldrich Fine Chemicals Biosciences CHES | 103-47-9 | MFCD00003835 | 1kg
CHES | Purity: 99% | Mol Wt: 207.29 | 103-47-9 | MFCD00003835 | 1kg
Sigma Aldrich Fine Chemicals Biosciences CHES BioXtra, >=99.0% (titration) | 103-47-9 | MFCD00003835 | 25G
CHES BioXtra, >=99.0% (titration) | Purity: >=99.0% (titration) | Mol Wt: 207.29 | 103-47-9 | MFCD00003835 | 25G
Sigma Aldrich Fine Chemicals Biosciences CAPS BioXtra, >=99% | 1135-40-6 | MFCD00003837 | 100G
CAPS BioXtra, >=99% | Purity: >=99% | Mol Wt: 221.32 | 1135-40-6 | MFCD00003837 | 100G
![Alkali Scientific DIPSO [3 (N,N Bis(2 Hydroxyethyl)amino) 2 hydroxypropane sulfonic acid], 25 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660391.jpg-250.jpg)
Alkali Scientific DIPSO [3 (N,N Bis(2 Hydroxyethyl)amino) 2 hydroxypropane sulfonic acid], 25 G
DIPSO (3 [N,N-Bis(2 Hydroxyethyl)amino] 2-hydroxypropane sulfonic acid) is a biochemical reagent available in a 25g bottle. This buffering agent is widely used in molecular biology, biochemistry, and analytical chemistry for maintaining stable pH levels in low pH environments. DIPSO is ideal for enzyme assays, protein research, and chromatography, where maintaining a precise pH is critical. It is also useful in protein purification and electrophoresis, providing consistent and reliable results. The 25g size is perfect for smaller scale experiments or routine laboratory applications where a high-quality buffering solution is necessary for pH regulation.
FUJIFILM Biosciences RPMI Medium 1640 1X HEPES
RPMI Medium 1640 1X HEPES is designed for growth of humanLeukemia cells in either monolayers or suspension and is useful for a wide variety of suspension and monolayer cultures as well as for hybridomas
![Alkali Scientific PIPES Sodium Salt [Piperazine N,N' bis(2 ethanesulfonic acid) sodium salt], 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659887.jpg-250.jpg)
Alkali Scientific PIPES Sodium Salt [Piperazine N,N' bis(2 ethanesulfonic acid) sodium salt], 500 Grams
PIPES Sodium Salt (Piperazine N,N' bis(2 ethanesulfonic acid) sodium salt) is a biochemical reagent used to create buffer solutions that maintain a stable pH environment. This 500g quantity is ideal for large-scale laboratory applications, such as enzyme reactions, protein chemistry, and molecular biology experiments. PIPES sodium salt buffers maintain a stable pH around 7.0, which is essential for preserving enzyme activity and protein integrity in biological assays. The high buffering capacity of PIPES sodium salt ensures pH consistency, making it a popular choice in cellular studies, microbiology, and biochemical research involving delicate biochemical reactions.